In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

نویسندگان

چکیده

The aim of this in vitro study was assessment the cytotoxic level 6-O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 exposed to naproxen and 6-O-desmethylnaproxen different concentrations for 24- 48- hours. Cell viability tested by MTT, while apoptosis determined AO/PI double staining method. control group free from any agent (DMEM only) accepted as 100% viability. IC50 results indicated that inhibition 50% resulted highest concentration (0.7 µg/mL) none caused dead. Consequently, avoid unacceptable side effects metabolites evacuated urine; further studies should be conducted determine accumulation metabolites.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Fibroblast involvement in soft connective tissue calcification

Soft connective tissue calcification is not a passive process, but the consequence of metabolic changes of local mesenchymal cells that, depending on both genetic and environmental factors, alter the balance between pro- and anti-calcifying pathways. While the role of smooth muscle cells and pericytes in ectopic calcifications has been widely investigated, the involvement of fibroblasts is stil...

متن کامل

Evaluation of Chronotropic Properties of Mouse Embryonic Stem Cells-Derived Cardiomyocytes After Fibroblast Growth Factor Treatment

Purpose: We investigated the effect of (bFGF) (basic-Fibroblast Growth Factor) on the differentiation of divided cardiomyocytes from mouse embryonic stem cells (ES) and their pharmacological properties. Materials and Methods: The mouse embryonic stem cells (Royan B1) were cultured as 800 cells per 20µl of a hanging drop. After two days, ES cells in each drop aggregated to form embryoid bodies ...

متن کامل

I-11: Dedifferentiation of Mouse Fibroblast Cells by Chemical Induction

Induced pluripotent stem cells (iPSCs) generated by ectopic expression of four transcription factors have great promises for regenerative medicine in humans. Since the initial report of iPSCs by viral transfection, ample efforts have been made in the generation of iPSCs through nonviral approaches. Small molecules offer the advantages of low cost without genomic modification and have been used ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Hacettepe Journal of Biology and Chemistry

سال: 2021

ISSN: ['1303-5002']

DOI: https://doi.org/10.15671/hjbc.680564